大矢 禎一 教授
Single cell phenomics of Saccharomyces cerevisiae is a powerful
approach to investigate strain-to-strain and cell-to-cell diversity
in an unbiased and statistical way. We applied this technique to
sake yeast strains to investigate their diversification during the
breeding process. Strain-to-strain comparison revealed that the sake
yeast population was diverse compared with S. cerevisiae in general.
We also observed an association between morphological profiles and
genotypes. Comparison with the lineage map revealed that cross breeding
had more profound effects on morphology than mutation breeding. Cell-
to-cell diversity revealed that robustness was perturbed in some sake
yeast strains, resulting in impairment of important intracellular
Thus, our high-dimensional, single-cell phenotyping provides valuable
information on the breeding of sake yeast, which will be useful for
future studies of breeding strategies in other microorganisms.