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Takuya Ueda / Professor / Division of Biosciences
Department of Computational Biololy and Medical Sciences /Laboratory of Biomolecules / Protein synthesis, Molecular evolution
http://park.itc.u-tokyo.ac.jp/molbio/uedalab.en/index.html

Career Summary
1979 Diploma of Agriculture, Department of Agricultural Chemistry , The University of Tokyo.
1981 Master D., Department of Agricultural Chemistry, The University of Tokyo.
1984 Ph.D., Department of Agricultural Chemistry, The University of Tokyo.
1984-1986: Postdoctoral-fellow, Max-Planck-Institut fur experimentelle Medizin, Germany.
1986-1988: Research Associate, Kihara Institute for Biological Research, Yokohama City University.
1988-1992: Research Associate, Department of Bioscience, Tokyo Institute of Technology.
1992-1994: Research Associate, Department of Chemistry and Biotechnology, The University of Tokyo.
1994-1997: Lecturer, Department of Chemistry and Biotechnology, The University of Tokyo.
1997-1999: Associate Professor, Department of Chemistry and Biotechnology, The University of Tokyo.
1999-2004: Professor, Department of Integrated Bioscience, The University of Tokyo.
2004-present: Professor, Department of Medical Genome Science, The University of Tokyo.

Educational Activities
Graduate School: Molecular Biology for Medical Science
Research Activities

1) Reconstitution of a gene expression system for protein research
We succeeded in reconstituting the E. coli translation system in the test tube. The system, which we named the PURE system, is capable of producing protein in one hour simply by adding the genetic message. I believe this system will provide a powerful platform technology for protein and proteome research in postgenome era. Based on this system, we are developing a system for producing membrane proteins by integrating membrane-targeting machinery and proteins with proper folding by supplementing molecular chaperones.

2) Creating polypeptides with new functions
The PURE system is also applicable for developing a method for identifying functional polypeptides from a pool of variants. We established the ribosome-display method using the PURE system. Based on this novel technique, we are creating various proteins with new binding properties.

3) Cross-talk between mitochondria and nucleus
Most mitochondrial proteins are encoded on nuclear DNA and are imported into the mitochondria from the cytoplasm. We are focusing on communication of gene expression between nucleus and mitochondria during cell differentiation and apoptosis. This basic research will allow development of therapeutic aspects of mitochondrial disease.
Literature
1) Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K. and Ueda, T. (2001) Cell-free translation reconstituted with purified components. Nat Biotechnol, 19, 751-755.

Other Activities
Japanese Society of Biochemistry, Japanese Society of Molecular Biology
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Future Plan
Life is orchestration of proteins. Synthetic biology or constructive biology is striving to assemble protein components necessary to create a biosystem in a test tube. Rapid production of proteins of high quality using the PURE system will largely contribute to expanding and establishing this new field of molecular biology.
Messages to Students
I hope that you will concentrate on research for the advancement of science and not for glory.

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