| Career Summary |
1984: Graduated, Faculty of Medicine, Kyushu University, M.D.
1987: Research Associate, Institute of Tropical Medicine, Nagasaki University
1990: Doctor of Medical Science from Kyushu University
1991: Postdoctoral Research Fellow, The University of California at Berkeley
1992: Research Associate, Institute of Medical Science, The University of Tokyo
1999: Associate Professor, Institute of Medical Science, The University of Tokyo
1999: Professor, Cancer Research Institute, Kanazawa University
2003: Professor, Graduate School of Frontier Sciences, The University of Tokyo
2009: Professor, Graduate School of Science, The University of Tokyo
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| Educational Activities |
Graduate school: Basic Bioinformatics II, Methods in Functional Genomics, Molecular Design Informatics
Undergraduate: Omic science
Other universities: Kyushu Univ.
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| Research Activities |
1. Yeast functional genomics/systems biology
How completely can we describe and understand the eukaryotic cell as a molecular system? To address this issue, we selected the budding yeast as a model and have conducted comprehensive analyses of its gene expression, protein-protein interactions and post-translational modifications.
For the transcriptome, we revealed its unexpected complexity generated by pervasive transcription of the yeast genome. We are currently trying to develop a more comprehensive method for quantitative transcriptome analysis as well as a strategy for discrimination between functional and non-functional transcripts.
For the proteome, our efforts are currently directed toward mass-spectrometry-based quantitative analysis of protein-protein interactions and post-translational modifications. We are also developing fluorescent sensor proteins for live cell imaging of key metabolites. We are planning to integrate these technologies to uncover mechanisms and regulatory principle of metabolic stress response.
2. Mammalian epigenomics
To achieve a comprehensive description of DNA methylation status at single-nucleotide resolution, we are exploiting new generation DNA sequencing technologies to establish a novel bisulfite shotgun sequencing method.
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Literature
1) Ito T. et al. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc. Natl. Acad. Sci. USA 98, 4569-4574, 2001.
2) Ito T. et al. Novel modular domain PB1 recognizes PC motif to mediate functional protein-protein interactions. EMBO J. 20, 3938-3946, 2001.
3) Yamada Y. et al. A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q. Genome Res. 14, 247-266, 2004.
4) Miura F. et al. A large-scale full-length cDNA analysis to explore the budding yeast transcriptome. Proc. Natl. Acad. Sci. USA 103, 17846-17851, 2006.
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| Other Activities |
Molecular Biology Society of Japan, The Japanese Biochemical Society, The Japanese Society for Epigenetics
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| Future Plan |
Comparative functional genomics/systems biology to reveal conservation and diversity of regulatory mechanisms for the understanding of their evolution
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| Messages to Students |
Carefully consider and pursue your own findings with diligence and patience.
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