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Kentaro Miyazaki / Professor /
Department of Computational Biology and Medical Sciences / / Functional Biomolecules Engineering
https://staff.aist.go.jp/miyazaki-kentaro/group/

Career Summary
1989: Graduated from Faculty of Science (Tokyo Institute of Technology)
1994: Received Ph.D. from Tokyo Institute of Technology
1994: Research Associate (National Institute of Advanced Industrial Science And Technology, AIST)
1997-2000: Visiting Associate (California Institute of Technology)
2000-2001: NEDO
2004-present: Group Leader (AIST)
2006-present: Adjunct Professor (University of Tokyo)
Educational Activities
Research Activities
Functional metagenomics (ref. 1-8)
Directed evolution (ref. 12-22)
Ribosome engineering (ref. 9-11)
Literature
1) Suenaga H., Koyama Y., Miyakoshi M., Miyazaki R., Yano H., Sota M., Ohtsubo Y., Tsuda M., and Miyazaki K. (2009) Novel organization of aromatic degradation pathway genes in a microbial community as revealed by metagenomic analysis. ISME J 3(12):1335-1348.
2) Suenaga H., Ohnuki T., and Miyazaki K. (2007) Functional screening of a metagenomic library for genes involved in microbial degradation of aromatic compounds. Environ Microbiol 9(9):2289-2297.
3) Uchiyama T., Yaoi K., and Miyazaki K. (2015) Glucose-tolerant -glucosidase retrieved from a Kusaya gravy metagenome. Front Microbiol 6:548. doi: 10.3389/fmicb.2015.00548.
4) Uchiyama T., Miyazaki K., and Yaoi K. (2013) Characterization of a novel -glucosidase from a compost microbial metagenome with strong transglycosylation activity. J Biol Chem 288(25):18325-18334.
5) Verma D., Kawarabayasi Y., Miyazaki K., and Satyanarayana T. (2013) Cloning, expression and characteristics of a novel alkalistable and thermostable xylanase encoding gene (mxyl) retrieved from compost-soil metagenome. PLOS ONE 8(1):e52459.
6) Uchiyama T. and Miyazaki K. (2013) Metagenomic screening for aromatic compound-responsive transcriptional regulators. PLOS ONE 8(9):e75795.
7) Uchiyama T. and Miyazaki K. (2010) Product-induced gene expression, a product-responsive reporter assay used to screen metagenomic libraries for enzyme-encoding genes. Appl Environ Microbiol 76(21):7029-7035.
8) Uchiyama T. and Miyazaki K. (2009) Functional metagenomics for enzyme discovery: challenges to efficient screening. Curr Opin Biotechnol 20(6):616-622.
9) Miyazaki K., Sato M., and Tsukuda M. (2017) PCR primer design for 16S rRNAs for experimental horizontal gene transfer test in Escherichia coli. Front. Bioeng. Biotechnol. 5:14. doi: 10.3389/fbioe.2017.00014
10) Kitahara K. and Miyazaki K. (2013) Revisiting bacterial phylogeny: Natural and experimental evidence for horizontal gene transfer of 16S rRNA. Mob Genet Elements 3(1):e24210.
11) Kitahara K., Yasutake Y., and Miyazaki K. (2012) Mutational robustness of 16S ribosomal RNA, shown by experimental horizontal gene transfer in Escherichia coli. Proc Natl Acad Sci USA 109(47):19220-19225.
12) Arnold F.H., Wintrode P.L., Miyazaki K., and Gershenson A. (2001) How enzymes adapt: lessons from directed evolution. Trends Biochem Sci 26(2)100-106.
13) Wintrode P.L., Miyazaki K., and Arnold F.H. (2000) Cold adaptation of a mesophilic subtilisin-like protease by directed evolution. J Biol Chem 275(41):31635-31640.
14) Miyazaki K., Wintrode P.L., Grayling R.A., Rubingh D.N., and Arnold F.H. (2000) Directed evolution study of temperature adaptation in a psychrophilic enzyme. J Mol Biol 297(4):1015-1026.
15) Miyazaki K. and Arnold F.H. (1999) Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function. J Mol Evol 49(6):716-720.
16) Tsukuda M. and Miyazaki K. (2013) Directed evolution study unveiling key sequence factors that affect translation efficiency in Escherichia coli. J Biosci Bioeng 116(5):540-545.
17) Yaoi K., Kondo H., Hiyoshi A., Noro N., Sugimoto H., Tsuda S., Mitsuishi Y., and Miyazaki K. (2007) Structure basis for the exo-mode of action in GH74 oligoxyloglucan reducing end-specific cellobiohydrolase. J Mol Biol 370(1):53-62.
18) Tsukuda M., Nakashima N., and Miyazaki K. (2015) Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli. J Biosci Bioeng 120(5):591-595.
19) Miyazaki K. (2015) Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58(2):86-88.
20) Miyazaki K. (2010) Lethal ccdB gene-based zero-background vector for construction of shotgun libraries. J Biosci Bioeng 110(3):372-373.
21) Miyazaki K. and Takenouchi M. (2002) Creating random mutagenesis libraries using megaprimer PCR of whole plasmid. Biotechniques 33(5):1033-1034, 1036-1038.
22) Miyazaki K. (2002) Random DNA fragmentation with endonuclease V: application to DNA shuffling. Nucleic Acids Res 30(24):e139.
Other Activities
Applied and Environmental Microbiology Editorial Board
Japan Society for Bioscience, Biotechnology, and Agrochemistry
The Society for Biotechnology, Japan
Japanese Society for Cell Synthesis Research
Japanese Society for Extremophiles
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Future Plan
Messages to Students
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